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gst-chmp4b ag4544  (Proteintech)


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    Structured Review

    Proteintech gst-chmp4b ag4544
    ecH2B binds and colocalizes with <t>CHMP4B</t> at the intercellular bridge. ( a ) GST-pull down was performed to analyze the binding between recombinant GST-CHMP4B and H2B. GST alone was used as negative control. WBs were performed to detect the indicated proteins. Red asterisks (*) indicate GST-CHMP4B sub-products consistently present in the commercial preparation. ( b ) Recombinant His-H2B was phosphorylated at Ser14 by incubation with HIPK2 Kinase Domain (HIPK2 KD) in the presence of cold γ-ATP. GST-pull down was performed to compare the binding between recombinant GST-CHMP4B and His-H2B or His-H2B-S14 P . GST alone was used as negative control. WBs were performed to detect the indicated proteins. Red asterisks (*) indicate GST-CHMP4B sub-products. ( c,d ) Proliferating, asynchronous HeLa cells were fixed and analyzed by proximity ligation assay (PLA) with anti-CHMP4B Ab and anti-phospho-H2B-Ser14 in two independent experiments ( c ) or anti-phospho-H2B-Ser32 Abs in two other independent experiments ( d ). Representative images of cells in early and late cytokinesis and abscission sites are shown. 30 ICBs per sample were analyzed and overall PLA positivity was observed in 37.7 ± 2.5% of the ICBs. Scale bar is 10 μm.
    Gst Chmp4b Ag4544, supplied by Proteintech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gst+chmp4b/anti+chmp4b/pmc06912571-61-3-8
    Average 90 stars, based on 1 article reviews
    gst-chmp4b ag4544 - by Bioz Stars, 2026-10
    90/100 stars

    Images

    1) Product Images from "Extrachromosomal Histone H2B Contributes to the Formation of the Abscission Site for Cell Division"

    Article Title: Extrachromosomal Histone H2B Contributes to the Formation of the Abscission Site for Cell Division

    Journal: Cells

    doi: 10.3390/cells8111391

    ecH2B binds and colocalizes with CHMP4B at the intercellular bridge. ( a ) GST-pull down was performed to analyze the binding between recombinant GST-CHMP4B and H2B. GST alone was used as negative control. WBs were performed to detect the indicated proteins. Red asterisks (*) indicate GST-CHMP4B sub-products consistently present in the commercial preparation. ( b ) Recombinant His-H2B was phosphorylated at Ser14 by incubation with HIPK2 Kinase Domain (HIPK2 KD) in the presence of cold γ-ATP. GST-pull down was performed to compare the binding between recombinant GST-CHMP4B and His-H2B or His-H2B-S14 P . GST alone was used as negative control. WBs were performed to detect the indicated proteins. Red asterisks (*) indicate GST-CHMP4B sub-products. ( c,d ) Proliferating, asynchronous HeLa cells were fixed and analyzed by proximity ligation assay (PLA) with anti-CHMP4B Ab and anti-phospho-H2B-Ser14 in two independent experiments ( c ) or anti-phospho-H2B-Ser32 Abs in two other independent experiments ( d ). Representative images of cells in early and late cytokinesis and abscission sites are shown. 30 ICBs per sample were analyzed and overall PLA positivity was observed in 37.7 ± 2.5% of the ICBs. Scale bar is 10 μm.
    Figure Legend Snippet: ecH2B binds and colocalizes with CHMP4B at the intercellular bridge. ( a ) GST-pull down was performed to analyze the binding between recombinant GST-CHMP4B and H2B. GST alone was used as negative control. WBs were performed to detect the indicated proteins. Red asterisks (*) indicate GST-CHMP4B sub-products consistently present in the commercial preparation. ( b ) Recombinant His-H2B was phosphorylated at Ser14 by incubation with HIPK2 Kinase Domain (HIPK2 KD) in the presence of cold γ-ATP. GST-pull down was performed to compare the binding between recombinant GST-CHMP4B and His-H2B or His-H2B-S14 P . GST alone was used as negative control. WBs were performed to detect the indicated proteins. Red asterisks (*) indicate GST-CHMP4B sub-products. ( c,d ) Proliferating, asynchronous HeLa cells were fixed and analyzed by proximity ligation assay (PLA) with anti-CHMP4B Ab and anti-phospho-H2B-Ser14 in two independent experiments ( c ) or anti-phospho-H2B-Ser32 Abs in two other independent experiments ( d ). Representative images of cells in early and late cytokinesis and abscission sites are shown. 30 ICBs per sample were analyzed and overall PLA positivity was observed in 37.7 ± 2.5% of the ICBs. Scale bar is 10 μm.

    Techniques Used: Binding Assay, Recombinant, Negative Control, Incubation, Proximity Ligation Assay

    ecH2B contributes to abscission site. ( a ) Proliferating, asynchronous HeLa cells were fixed and analyzed by double IF with anti-β-Tubulin Ab (red) and anti-phospho-H2B-Ser14 Ab (green) in more than five independent experiments. Representative images of early and late cytokinesis and abscission sites are shown. Fifty ICBs per sample were analyzed. Scale bar is 10 μm. ( b ) Proliferating, asynchronous HeLa cells were fixed and analyzed by double IF with anti-phospho-H2B-Ser14 Ab (green) and anti-CHMP4B Ab. Two representative images for late cytokinesis, abscission site and the relative 3D reconstructions are shown. ( c , d ) CTRi and H2Bi HeLa cells were obtained as above and analyzed by double IF for β-Tubulin (red) and the indicated proteins (green); DNA was marked with Hoechst (Blue). Representative images ( c ) and quantification ( d ) from three independent experiments of abscission site formation based on the three abscission factors are reported. Data are reported as mean ± SD. ** p < 0.01; *** p < 0.001. ( e ) Graphical abstract is reported. Microtubules are indicated in red, ecH2B is represented in blue and CHMP4B in green.
    Figure Legend Snippet: ecH2B contributes to abscission site. ( a ) Proliferating, asynchronous HeLa cells were fixed and analyzed by double IF with anti-β-Tubulin Ab (red) and anti-phospho-H2B-Ser14 Ab (green) in more than five independent experiments. Representative images of early and late cytokinesis and abscission sites are shown. Fifty ICBs per sample were analyzed. Scale bar is 10 μm. ( b ) Proliferating, asynchronous HeLa cells were fixed and analyzed by double IF with anti-phospho-H2B-Ser14 Ab (green) and anti-CHMP4B Ab. Two representative images for late cytokinesis, abscission site and the relative 3D reconstructions are shown. ( c , d ) CTRi and H2Bi HeLa cells were obtained as above and analyzed by double IF for β-Tubulin (red) and the indicated proteins (green); DNA was marked with Hoechst (Blue). Representative images ( c ) and quantification ( d ) from three independent experiments of abscission site formation based on the three abscission factors are reported. Data are reported as mean ± SD. ** p < 0.01; *** p < 0.001. ( e ) Graphical abstract is reported. Microtubules are indicated in red, ecH2B is represented in blue and CHMP4B in green.

    Techniques Used:

    Related Articles

    Binding Assay:

    Article Title: Extrachromosomal Histone H2B Contributes to the Formation of the Abscission Site for Cell Division
    Article Snippet: .. For H2B and CHMP4B binding assays, GST-CHMP4B (ag4544, Proteintech, Rosemont, IL, USA) was incubated overnight at room temperature with 500 ng of recombinant His-H2B (ag7811, Proteintech) or histone H2B (#14-491, Millipore) in buffer phosphate pH 7.5, 150 mM NaCl. .. GST-pulldown was performed by incubation for 2 h at 4 °C with Glutathione-Sepharose 4 Fast Flow beads (GE Healthcare, Buckinghamshire, UK) and three washes with buffer phosphate.

    Incubation:

    Article Title: Extrachromosomal Histone H2B Contributes to the Formation of the Abscission Site for Cell Division
    Article Snippet: .. For H2B and CHMP4B binding assays, GST-CHMP4B (ag4544, Proteintech, Rosemont, IL, USA) was incubated overnight at room temperature with 500 ng of recombinant His-H2B (ag7811, Proteintech) or histone H2B (#14-491, Millipore) in buffer phosphate pH 7.5, 150 mM NaCl. .. GST-pulldown was performed by incubation for 2 h at 4 °C with Glutathione-Sepharose 4 Fast Flow beads (GE Healthcare, Buckinghamshire, UK) and three washes with buffer phosphate.

    Recombinant:

    Article Title: Extrachromosomal Histone H2B Contributes to the Formation of the Abscission Site for Cell Division
    Article Snippet: .. For H2B and CHMP4B binding assays, GST-CHMP4B (ag4544, Proteintech, Rosemont, IL, USA) was incubated overnight at room temperature with 500 ng of recombinant His-H2B (ag7811, Proteintech) or histone H2B (#14-491, Millipore) in buffer phosphate pH 7.5, 150 mM NaCl. .. GST-pulldown was performed by incubation for 2 h at 4 °C with Glutathione-Sepharose 4 Fast Flow beads (GE Healthcare, Buckinghamshire, UK) and three washes with buffer phosphate.



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    gst-chmp4b ag4544  (Proteintech)
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    ecH2B binds and colocalizes with <t>CHMP4B</t> at the intercellular bridge. ( a ) GST-pull down was performed to analyze the binding between recombinant GST-CHMP4B and H2B. GST alone was used as negative control. WBs were performed to detect the indicated proteins. Red asterisks (*) indicate GST-CHMP4B sub-products consistently present in the commercial preparation. ( b ) Recombinant His-H2B was phosphorylated at Ser14 by incubation with HIPK2 Kinase Domain (HIPK2 KD) in the presence of cold γ-ATP. GST-pull down was performed to compare the binding between recombinant GST-CHMP4B and His-H2B or His-H2B-S14 P . GST alone was used as negative control. WBs were performed to detect the indicated proteins. Red asterisks (*) indicate GST-CHMP4B sub-products. ( c,d ) Proliferating, asynchronous HeLa cells were fixed and analyzed by proximity ligation assay (PLA) with anti-CHMP4B Ab and anti-phospho-H2B-Ser14 in two independent experiments ( c ) or anti-phospho-H2B-Ser32 Abs in two other independent experiments ( d ). Representative images of cells in early and late cytokinesis and abscission sites are shown. 30 ICBs per sample were analyzed and overall PLA positivity was observed in 37.7 ± 2.5% of the ICBs. Scale bar is 10 μm.
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    gst chmp4b  (Proteintech)
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    ecH2B binds and colocalizes with <t>CHMP4B</t> at the intercellular bridge. ( a ) GST-pull down was performed to analyze the binding between recombinant GST-CHMP4B and H2B. GST alone was used as negative control. WBs were performed to detect the indicated proteins. Red asterisks (*) indicate GST-CHMP4B sub-products consistently present in the commercial preparation. ( b ) Recombinant His-H2B was phosphorylated at Ser14 by incubation with HIPK2 Kinase Domain (HIPK2 KD) in the presence of cold γ-ATP. GST-pull down was performed to compare the binding between recombinant GST-CHMP4B and His-H2B or His-H2B-S14 P . GST alone was used as negative control. WBs were performed to detect the indicated proteins. Red asterisks (*) indicate GST-CHMP4B sub-products. ( c,d ) Proliferating, asynchronous HeLa cells were fixed and analyzed by proximity ligation assay (PLA) with anti-CHMP4B Ab and anti-phospho-H2B-Ser14 in two independent experiments ( c ) or anti-phospho-H2B-Ser32 Abs in two other independent experiments ( d ). Representative images of cells in early and late cytokinesis and abscission sites are shown. 30 ICBs per sample were analyzed and overall PLA positivity was observed in 37.7 ± 2.5% of the ICBs. Scale bar is 10 μm.
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    Image Search Results


    ecH2B binds and colocalizes with CHMP4B at the intercellular bridge. ( a ) GST-pull down was performed to analyze the binding between recombinant GST-CHMP4B and H2B. GST alone was used as negative control. WBs were performed to detect the indicated proteins. Red asterisks (*) indicate GST-CHMP4B sub-products consistently present in the commercial preparation. ( b ) Recombinant His-H2B was phosphorylated at Ser14 by incubation with HIPK2 Kinase Domain (HIPK2 KD) in the presence of cold γ-ATP. GST-pull down was performed to compare the binding between recombinant GST-CHMP4B and His-H2B or His-H2B-S14 P . GST alone was used as negative control. WBs were performed to detect the indicated proteins. Red asterisks (*) indicate GST-CHMP4B sub-products. ( c,d ) Proliferating, asynchronous HeLa cells were fixed and analyzed by proximity ligation assay (PLA) with anti-CHMP4B Ab and anti-phospho-H2B-Ser14 in two independent experiments ( c ) or anti-phospho-H2B-Ser32 Abs in two other independent experiments ( d ). Representative images of cells in early and late cytokinesis and abscission sites are shown. 30 ICBs per sample were analyzed and overall PLA positivity was observed in 37.7 ± 2.5% of the ICBs. Scale bar is 10 μm.

    Journal: Cells

    Article Title: Extrachromosomal Histone H2B Contributes to the Formation of the Abscission Site for Cell Division

    doi: 10.3390/cells8111391

    Figure Lengend Snippet: ecH2B binds and colocalizes with CHMP4B at the intercellular bridge. ( a ) GST-pull down was performed to analyze the binding between recombinant GST-CHMP4B and H2B. GST alone was used as negative control. WBs were performed to detect the indicated proteins. Red asterisks (*) indicate GST-CHMP4B sub-products consistently present in the commercial preparation. ( b ) Recombinant His-H2B was phosphorylated at Ser14 by incubation with HIPK2 Kinase Domain (HIPK2 KD) in the presence of cold γ-ATP. GST-pull down was performed to compare the binding between recombinant GST-CHMP4B and His-H2B or His-H2B-S14 P . GST alone was used as negative control. WBs were performed to detect the indicated proteins. Red asterisks (*) indicate GST-CHMP4B sub-products. ( c,d ) Proliferating, asynchronous HeLa cells were fixed and analyzed by proximity ligation assay (PLA) with anti-CHMP4B Ab and anti-phospho-H2B-Ser14 in two independent experiments ( c ) or anti-phospho-H2B-Ser32 Abs in two other independent experiments ( d ). Representative images of cells in early and late cytokinesis and abscission sites are shown. 30 ICBs per sample were analyzed and overall PLA positivity was observed in 37.7 ± 2.5% of the ICBs. Scale bar is 10 μm.

    Article Snippet: For H2B and CHMP4B binding assays, GST-CHMP4B (ag4544, Proteintech, Rosemont, IL, USA) was incubated overnight at room temperature with 500 ng of recombinant His-H2B (ag7811, Proteintech) or histone H2B (#14-491, Millipore) in buffer phosphate pH 7.5, 150 mM NaCl.

    Techniques: Binding Assay, Recombinant, Negative Control, Incubation, Proximity Ligation Assay

    ecH2B contributes to abscission site. ( a ) Proliferating, asynchronous HeLa cells were fixed and analyzed by double IF with anti-β-Tubulin Ab (red) and anti-phospho-H2B-Ser14 Ab (green) in more than five independent experiments. Representative images of early and late cytokinesis and abscission sites are shown. Fifty ICBs per sample were analyzed. Scale bar is 10 μm. ( b ) Proliferating, asynchronous HeLa cells were fixed and analyzed by double IF with anti-phospho-H2B-Ser14 Ab (green) and anti-CHMP4B Ab. Two representative images for late cytokinesis, abscission site and the relative 3D reconstructions are shown. ( c , d ) CTRi and H2Bi HeLa cells were obtained as above and analyzed by double IF for β-Tubulin (red) and the indicated proteins (green); DNA was marked with Hoechst (Blue). Representative images ( c ) and quantification ( d ) from three independent experiments of abscission site formation based on the three abscission factors are reported. Data are reported as mean ± SD. ** p < 0.01; *** p < 0.001. ( e ) Graphical abstract is reported. Microtubules are indicated in red, ecH2B is represented in blue and CHMP4B in green.

    Journal: Cells

    Article Title: Extrachromosomal Histone H2B Contributes to the Formation of the Abscission Site for Cell Division

    doi: 10.3390/cells8111391

    Figure Lengend Snippet: ecH2B contributes to abscission site. ( a ) Proliferating, asynchronous HeLa cells were fixed and analyzed by double IF with anti-β-Tubulin Ab (red) and anti-phospho-H2B-Ser14 Ab (green) in more than five independent experiments. Representative images of early and late cytokinesis and abscission sites are shown. Fifty ICBs per sample were analyzed. Scale bar is 10 μm. ( b ) Proliferating, asynchronous HeLa cells were fixed and analyzed by double IF with anti-phospho-H2B-Ser14 Ab (green) and anti-CHMP4B Ab. Two representative images for late cytokinesis, abscission site and the relative 3D reconstructions are shown. ( c , d ) CTRi and H2Bi HeLa cells were obtained as above and analyzed by double IF for β-Tubulin (red) and the indicated proteins (green); DNA was marked with Hoechst (Blue). Representative images ( c ) and quantification ( d ) from three independent experiments of abscission site formation based on the three abscission factors are reported. Data are reported as mean ± SD. ** p < 0.01; *** p < 0.001. ( e ) Graphical abstract is reported. Microtubules are indicated in red, ecH2B is represented in blue and CHMP4B in green.

    Article Snippet: For H2B and CHMP4B binding assays, GST-CHMP4B (ag4544, Proteintech, Rosemont, IL, USA) was incubated overnight at room temperature with 500 ng of recombinant His-H2B (ag7811, Proteintech) or histone H2B (#14-491, Millipore) in buffer phosphate pH 7.5, 150 mM NaCl.

    Techniques: